Insect disinfestation in smoked fish by gamma irradiation

The experiment on insect disinfestation in smoked fish by gamma irradiation was done by irradiating 2-day-old eggs, 28-day-old larvae, 5-day-old pupae, and 5-day-old adults, reared at 26 +- 1 degC and 75-70% RH., with doses of 0-.5, 0-20 Krad respectively for sterility dose and with doses of 0-2, 0-6, 0-60, 20-60 Krad respectively for LD50. The results obtained from this experiment are as follows: (1) For the study of all stages of this insect sterility doses, there was no irradiated egg hatch in all doses and only irradiated larvae with 1-5 Krad reached pupal stage but emerged as malformed adults and died. Furthermore, the sterility doses for pupal and adult stage are both at 7.5 Krad. However, the percent of egg hatch from 5 Krad pupae and 5 Krad adults are .18 and .4 respectively. (2) The LD50 checked at different times after irradiation in each stage for 2-day-old eggs after 2 days, for 28-day-old larvae after 7 days, 5-day-old pupae after 5 days, and for 5-day-old adults after 5 days are .48, 12.85, 18.5 and 29.78 Krad respectively