125I-urokinase radioimmunoassay
- 1. Shanghai Medical Univ., SH (China)
Description
A sensitive 125I-urokinase (UK) radioimmunoassay (RIA) is described. 125I-UK was prepared by the chloramine T method. Its specific radioactivity was 14.8 x 105 Bq/μg. The antiserum against UK was raised by immunizing healthy New Zealand white rabbits with a mixture of UK and Freund's complete adjuvant. A titer of antiserum at 1:2000, and an affinity constant of 1.62 x 1010 L/mol, was specific for UK. The mean recoveries in serum and urine were 99.4 ± 5.0% and 101.1 ± 7.4%, respectively. THe value of coefficient of variation, intraassay and interassay were both less than 10%. The low detection limit for UK antigen was 0.18 ng. The detection range was 0.38 ∼ 12.16 ng/tube. Serum and urine UK levels in normal controls were 6.37 ± 1.28 ng/ml (n = 74) and 39.30 ± 18.04 ng/ml (n = 49), respectively
Additional details
Publishing Information
- Journal Title
- Acta Academiae Medicinae Shanghai
- Journal Volume
- 8
- Journal Issue
- 2
- Series
- Acta Acad. Med. Shanghai.
- Journal Page Range
- 443-446
- ISSN
- 0257-8131
- CODEN
- SYDXE
INIS
- Country of Publication
- China
- Country of Input or Organization
- China
- INIS RN
- 22041902
- Subject category
- S38: RADIATION CHEMISTRY, RADIOCHEMISTRY AND NUCLEAR CHEMISTRY;
- Descriptors DEI
- BLOOD SERUM; IODINE 125; LABELLED COMPOUNDS; LABELLING; RADIOIMMUNODETECTION; SAMPLE PREPARATION; UROKINASE
- Descriptors DEC
- BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD COAGULATION FACTORS; BLOOD PLASMA; BODY FLUIDS; COAGULANTS; DAYS LIVING RADIOISOTOPES; DIAGNOSTIC TECHNIQUES; DRUGS; ELECTRON CAPTURE RADIOISOTOPES; ENZYMES; HEMATOLOGIC AGENTS; HYDROLASES; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTO; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MATERIALS; NONSPECIFIC PEPTIDASES; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; PEPTIDE HYDROLASES; RADIOISOTOPES; TRACER TECHNIQUES