The protective effects of glycerine on mammalian cells in ion implantation

The discovery of bio-effects induced by ion implantation has opened a novel branch in the field of radiation biology-the low energy heavy ion biology. The experimental samples in the field have, however, been within the limits of plants and microbes because of the sample's vacuum-resistant ability. The mutation studying of mammalian cells with ion implantation hasn't been reported in the past years. Main reason for that is that it is difficult to keep mammalian cells alive in vacuum during ion implantation. In the present study, the tolerance of mammalian cells in vacuum was enhanced efficiently by pre-treatment with glycerin at low concentration and proper treatment time, which provide a new way for the study on the biological effects of ion beam implantation in the mammalian cells