Synthesis of adinazolam mesylate multiply labeled with carbon-13 and deuterium

Adinazolam mesylate an anxiolytic and antidepressant, labelled with stable isotopes was synthesized for conducting bioavailability studies. Adinazolam labelled uniformly with carbon-13, > 99% enrichment, in the unsubstituted phenyl ring was prepared for administration as drug. In addition, adinazolam was also dual-labelled with carbon-13, as in the drug, and with deuterium, six atoms per molecule, in the N-methyl groups. The double-labelled material was prepared for use as an internal standard in the mass spectral assay procedure to be employed in the study. (author)