Published July 30, 2010 | Version v1
Journal article Metadata-only

Synthesis of CdTe/CdS/ZnS quantum dots and their application in imaging of hepatocellular carcinoma cells and immunoassay for alpha fetoprotein

Creators

  • 1. Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), College of Chemistry and Chemical Engineering of Guangxi Normal University, Guilin 541004 (China)
  • 2. Pharmacology Department of Guilin Medical College, Guilin 541004 (China)

Description

We report the imaging of hepatocellular carcinoma cells and the immunoassay for alpha fetoprotein (AFP) using CdTe/CdS/ZnS core-shell-shell QDs. Stable and high PLQY (20%-48%) CdTe/CdS/ZnS core-shell-shell QDs were synthesized by a stepwise process. Bioconjugation of the core-shell-shell QDs with streptavidin (SA) was successfully applied in immunofluorescent imaging of the human hepatocellular carcinoma (HCC) cell line HepG2.2.15. Furthermore, the thioglycolic acid (TGA)-capped CdTe/CdS/ZnS core-shell-shell QDs fluorescence lifetime is longer than fluorescein, so it was first engaged to conjugate with antigen for the determination of protein (AFP) by fluorescence polarization immunoassay.

Availability note (English)

Available from http://dx.doi.org/10.1088/0957-4484/21/30/305101

Additional details

Identifiers

DOI
10.1088/0957-4484/21/30/305101;
PII
S0957-4484(10)50250-2;

Publishing Information

Journal Title
Nanotechnology (Print)
Journal Volume
21
Journal Issue
30
Journal Page Range
[8 p.]
ISSN
0957-4484

INIS

Country of Publication
United Kingdom
Country of Input or Organization
International Atomic Energy Agency (IAEA)
INIS RN
43025015
Subject category
S77: NANOSCIENCE AND NANOTECHNOLOGY;
Descriptors DEI
ANTIGENS; CADMIUM SULFIDES; CADMIUM TELLURIDES; FLUORESCEIN; FLUORESCENCE; HEPATOMAS; IMMUNOASSAY; LIFETIME; PROTEINS; QUANTUM DOTS; SHELLS; SYNTHESIS; ZINC SULFIDES
Descriptors DEC
AROMATICS; BIOASSAY; CADMIUM COMPOUNDS; CARBOXYLIC ACIDS; CARCINOMAS; CHALCOGENIDES; DISEASES; DYES; EMISSION; HYDROXY ACIDS; HYDROXY COMPOUNDS; INORGANIC PHOSPHORS; LUMINESCENCE; NANOSTRUCTURES; NEOPLASMS; ORGANIC ACIDS; ORGANIC COMPOUNDS; PHENOLS; PHOSPHORS; PHOTON EMISSION; POLYPHENOLS; SULFIDES; SULFUR COMPOUNDS; TELLURIDES; TELLURIUM COMPOUNDS; ZINC COMPOUNDS