D1 protein of Drosophila melanogaster. Purification and AT-DNA binding properties
Description
D1 protein of Drosophila melanogaster is a sequence-specific DNA-binding protein which recognizes AT-rich DNA sequences. AT-rich DNA sequences in eukaryotic organisms are distributed in two characteristic ways: flanking transcriptional units and in constitutive heterochromatin. D1 could play a role in regulation of gene expression and in geographical localization of DNA sequences within the nucleus. D1 has been partially purified by ion exchange chromatography. DNA-binding activity was investigated by nucleoprotein gel electrophoresis, using end-labeled restriction fragments varying in AT sequence content. D1 binds most tightly to the satellite sequence -AATAT-, with intermediate strength to the complex satellite (359-base pair repeat) and another AT-rich (68% AT) mixed sequence DNA, and least to the simple satellite sequence -AAGAG-
Additional details
Publishing Information
- Journal Title
- J. Biol. Chem.
- Journal Issue
- no.26
- Series
- J. Biol. Chem.
- ISSN
- 0021-9258
- CODEN
- JBCHA
INIS
- Country of Publication
- United States
- Country of Input or Organization
- United States
- INIS RN
- 17043637
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- BIOCHEMICAL REACTION KINETICS; CELL CULTURES; CHEMICAL BONDS; CHROMATOGRAPHY; DNA; DNA SEQUENCING; DROSOPHILA; ELECTROPHORESIS; ION EXCHANGE; NUCLEOPROTEINS; PURIFICATION; RADIOISOTOPES; SOLUBILITY; THYMINE
- Descriptors DEC
- ANIMALS; ARTHROPODS; AZINES; FLIES; FRUIT FLIES; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; INSECTS; INVERTEBRATES; ISOTOPES; KINETICS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PROTEINS; PYRIMIDINES; REACTION KINETICS; SEPARATION PROCESSES; STRUCTURAL CHEMICAL ANALYSIS; URACILS