Published 1978 | Version v1
Journal article Metadata-only

Unscheduled DNA-synthesis in UV-irradiated spleen lymphocytes of the rat after whole-body X-irradiation

Creators

  • 1. Muenchen Univ. (Germany, F.R.). Inst. fuer Pharmakologie, Toxikologie und Pharmazie
  • 2. Muenchen Univ. (Germany, F.R.). Fachbereich Tiermedizin

Description

Unscheduled DNA-synthesis of UV-irradiated spleen lymphocytes of the rat is inhibited in a dose-dependent manner by total X-irradiation. The reaction is observed immediately after exposure and reaches its maximum within 8-16 h. Doses of some rads are detectable. Following sublethal X-irradiation (250 rad), regeneration takes 4-6 weeks. Analogous results have been obtained in rats treated with 2,4,6-triethyleneimino-1,3,5-triazine. Regardless of the action mechanisms, it is concluded from the present results that the inhibition of the unscheduled incorporation of thymidine-3H in splenic lymphocytes is a very sensitive, though not specific expression of impairment of the lymphatic systems. (orig.)

Abstract (German)

Die ausserplanmaessige DNS-Synthese UV-bestrahlter Rattenmilz-Lymphozyten wird durch Ganzkoerper-Roentgenbestrahlung dosisabhaengig gehemmt. Die Reaktion setzt unmittelbar nach der Strahlenexposition ein und erreicht nach 8-16 h ein Maximum. Dosen von einigen rad sind nachweisbar. Nach subletaler Bestrahlung (250 rad) dauert die Regenerationszeit 4-6 Wochen. Analoge Ergebnisse erhielt man mit Ratten, die mit 2,4,6-Triaethylenimino-1,3,5-triazin behandelt wurden. Unabhaengig von den Wirkungsmechanismen gelangt man zu dem Schluss, dass die Hemmung der ausserplanmaessigen Inkorporierung von 3H-Thymidin in Milz-Lymphozyten ein sehr empfindliches, jedoch nicht spezifisches Anzeichen fuer eine Hemmung des lymphatischen Systems darstellt. (orig.)

Additional details

Identifiers

DOI
10.1007/bf01330901;

Publishing Information

Journal Title
Radiation and Environmental Biophysics
Journal Volume
15
Journal Issue
1
Series
Radiat. Environ. Biophys.
Journal Page Range
77-83
ISSN
0301-634X

INIS

Country of Publication
Germany
Country of Input or Organization
Germany
INIS RN
9391694
Subject category
S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
Descriptors DEI
BIOLOGICAL EFFECTS; BIOLOGICAL RADIATION EFFECTS; BIOLOGICAL REGENERATION; BIOSYNTHESIS; DNA; LYMPHOCYTES; RATS; SPLEEN; SUBLETHAL IRRADIATION; THYMIDINE; TIME DEPENDENCE; TRACER TECHNIQUES; TRIAZINES; TRITIUM COMPOUNDS; ULTRAVIOLET RADIATION; UPTAKE; WHOLE-BODY IRRADIATION; X RADIATION
Descriptors DEC
ANIMAL CELLS; ANIMALS; AZINES; BIOLOGICAL MATERIALS; BIOLOGICAL RECOVERY; BLOOD; BLOOD CELLS; BODY; BODY FLUIDS; CONNECTIVE TISSUE CELLS; ELECTROMAGNETIC RADIATION; EXTERNAL IRRADIATION; HETEROCYCLIC COMPOUNDS; HYDROGEN COMPOUNDS; IONIZING RADIATIONS; IRRADIATION; ISOTOPE APPLICATIONS; LEUKOCYTES; MAMMALS; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; PYRIMIDINES; RADIATION EFFECTS; RADIATIONS; RIBOSIDES; RODENTS; SOMATIC CELLS; SYNTHESIS; VERTEBRATES

Optional Information

Notes
4 figs.; 21 refs.; Updated automatically by Metadata and Full-Text Enrichment Agent