Mechanism of ochratoxin A transport in kidney
Creators
- 1. State Univ. of New York, Syracuse (USA)
Description
The effect of the fungal metabolite (mycotoxin) Ochratoxin A (OTA) on the transport of p-amino[3H]hippurate (PAH), a prototypic organic anion, was examined in renal brush border (BBMV) and basolateral membrane vesicles (BLMV). OTA was as effective an inhibitor of PAH uptake in both membranes as probenecid. The dose response curves for OTA in BBMV and BLMV gave IC50 values of 20 +/- 6 and 32 +/- 7 microM, respectively. The effect was specific since the transport of the organic cation N1-methylnicotinamide was not affected. The phenomenon of counterflow was studied to establish that OTA is translocated. OTA produced trans stimulation of PAH transport in both BBMV and BLMV, demonstrating that OTA is transported across both these membranes. The data suggest that OTA interacts with the PAH transport system in both BBMV and BLMV. We conclude that OTA transport in the kidney is mediated via the renal organic anion transport system
Additional details
Publishing Information
- Journal Title
- Journal of Pharmacology and Experimental Therapeutics
- Journal Volume
- 246
- Journal Issue
- 2
- Series
- J. Pharmacol. Exp. Ther.
- Journal Page Range
- 460-465
- ISSN
- 0022-3565
- CODEN
- JPETA
INIS
- Country of Publication
- United States
- Country of Input or Organization
- United States
- INIS RN
- 20005796
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- ANIONS; ASPERGILLUS; CELL MEMBRANES; DOGS; IN VITRO; KIDNEYS; MEMBRANE TRANSPORT; METABOLITES; NICOTINAMIDE; TOXINS; TRACER TECHNIQUES; TRITIUM COMPOUNDS
- Descriptors DEC
- AMIDES; ANIMALS; ANTIGENS; AZINES; BODY; CELL CONSTITUENTS; CHARGED PARTICLES; FUNGI; HETEROCYCLIC COMPOUNDS; HYDROGEN COMPOUNDS; IONS; ISOTOPE APPLICATIONS; MAMMALS; MEMBRANES; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; PLANTS; PYRIDINES; VERTEBRATES; VITAMIN B GROUP; VITAMINS