Published November 1992 | Version v1
Journal article Metadata-only

A study on the immuno-activity of 211At-anti-gastric cancer McAb3H11 and its injury effect on gastric cancer cells

Creators

  • 1. Institute of Nuclear Science and Technology, Sichuan Univ. (China)
  • 2. Beijing Cancer Institute (China)

Description

The synthesis of 211At labelled 3H11 monoclonal antibody was performed by an activation reaction of carboxy via para-astato-benzoic acid intermediate. Its specific radioactivity was 18.5-61.1 kBq/μg. Its immuno-activity was measured by ELISA method. In vitro binding and cytotoxic experiments showed when the 2112At-3H11McAb concentration is raised, the cytotoxic effect increased and have obvious selectivity. The cytotoxic effect of 211At-3H11McAb to target cell M85 was higher than that of 211At-IgG and Na211At. If the M85 cells were cultured with 3H11McAb or 3G9McAb for 1 h at first, the cytotoxic effect of 211At-3H11McAb was inhibited. The specific radioactivity of 211At-3H11McAb and cytotoxic effect are also discussed

Additional details

Publishing Information

Journal Title
Nuclear Techniques
Journal Volume
15
Journal Issue
11
Journal Page Range
p. 678-682.
ISSN
0253-3219
CODEN
NUTEDL

INIS

Country of Publication
China
Country of Input or Organization
China
INIS RN
24056349
Subject category
S38: RADIATION CHEMISTRY, RADIOCHEMISTRY AND NUCLEAR CHEMISTRY; S62: RADIOLOGY AND NUCLEAR MEDICINE;
Descriptors DEI
ASTATINE 211; IMMUNITY; IMMUNOASSAY; LABELLED COMPOUNDS; LABELLING; MONOCLONAL ANTIBODIES; MORTALITY; TUMOR CELLS
Descriptors DEC
ALPHA DECAY RADIOISOTOPES; ANIMAL CELLS; ANTIBODIES; ASTATINE ISOTOPES; BETA DECAY RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; HEAVY NUCLEI; HOURS LIVING RADIOISOTOPES; ISOTOPES; NUCLEI; ODD-EVEN NUCLEI; RADIOISOTOPES