Effects of space mutation on P(T) GMS-line Pei'ai 64S of rice and SSR analysis of mutants

Some traits of the progenies SP1, SP2 and SP3 derived from Pei'ai 64S treated by space flight of recoverable satellite were studied, and polymorphism analysis of SSR for the mutants were carried out. The results showed that no difference between the treated Pei'ai 64S and the original one in germination rate, survival seedling rate, plant height, heading data and plant type in SP1 was observed, but there was significant difference in fertility, plant height and grain shape in generation of SP2. Some mutants with much bigger stigma than that of the original Pei'ai 64S were obtained in SP3, and it would be useful in improving the out-crossing rate and enhancing the seed production of Pei'ai 64S. In addition, SSR marker analysis for ten mutants indicated that the average mutant frequency of SSR loci for the mutants was 23.33% and the mutant loci randomly distributed along the chromosomes. Among the mutant SSR loci about 55.21% showed changes in band number and 44.78% in molecular weight. DNA deficiency and duplication might be the main reason of mutation caused by space flight. (authors)