Published 2006 | Version v1
Miscellaneous Restricted

Application of PCC-FISH method for biological dosimetry

Creators

  • 1. Inst. for Environmental Sciences, Dept. of Radioecology, Rokkasho, Aomori (Japan)

Description

Dicentric chromosome aberration of lymphocytes is widely used as a sensitivity marker for biological dosimetry. We established a precise and sensitive PCC method that combines premature chromosome condensation (PCC) and fluorescence in situ hybridization (FISH) to detect dicentric chromosomes. Human peripheral lymphocytes were irradiated in vitro with 1 to 5 Gy of X-ray. Then metaphase preparations were made using PCC to examine dicentric chromosomes using FISH with a centromere specific probe. Yields of dicentric chromosomes by PCC-FISH were about two times higher than those obtained using conventional Giemsa staining. (author)

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Part of:
Annual report of the Institute for Environmental Sciences, 2004

Additional details

Additional titles

Original title (Japanese)
Heisei 16-nendo kankyo kagaku gijutsu kenkyusho nenpo

Publishing Information

Imprint Title
Annual report of the Institute for Environmental Sciences, 2004
Imprint Pagination
141 p.
Journal Page Range
p. 73-74
Report number
INIS-JP--098

INIS

Country of Publication
Japan
Country of Input or Organization
Japan
INIS RN
38109218
Subject category
S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
Descriptors DEI
BIOLOGY; CHROMOSOMAL ABERRATIONS; DETECTION; DICENTRIC CHROMOSOMES; DOSIMETRY; FLUORESCENCE; HYBRID SYSTEMS; IN-SITU HYBRIDIZATION; LYMPHOCYTES; MITOSIS; RADIATION DOSES; X RADIATION; YIELDS
Descriptors DEC
ANIMAL CELLS; BIOLOGICAL MATERIALS; BIOTECHNOLOGY; BLOOD; BLOOD CELLS; BODY FLUIDS; CELL DIVISION; CHROMOSOMES; CONNECTIVE TISSUE CELLS; DOSES; ELECTROMAGNETIC RADIATION; EMISSION; GENETIC ENGINEERING; IONIZING RADIATIONS; LEUKOCYTES; LUMINESCENCE; MATERIALS; MUTATIONS; NUCLEIC ACID HYBRIDIZATION; PHOTON EMISSION; RADIATIONS; SOMATIC CELLS

Optional Information

Notes
2 refs., 2 figs. Imprint:Heisei 16-nendo kankyo kagaku gijutsu kenkyusho nenpo