Development of new radioactive labelling methods (3H and 11C) in luteizing hormone (LH) and its releasing hormone (LRF). Study of physico-chemical properties of LRF by circular dichroism and emission spectroscopy
Creators
Description
After a brief review of present knowledge on the hypothalamus-hypophysis this thesis falls into three parts. The first situates the peptide hormones studied in their biological context. Research on the radioactive labelling of hormonal peptides is dealt with in part two which includes, besides the application of already known tritiation methods to particular problems, the description of a new tritium labelling method and the use of carbon 11 for the kinetic distribution study of a hormone. Part three concerns the physico-chemical study of a hypothalamic hormone. As a contribution towards research on the hypophysary gonadotrophic function regulation, the work involved in all the above three sections was directed towards the luteinising hormone (LH) and its hypothalamic release factor (LRF). During the study of this latter the problem of peptides containing tryptophane arose and was consequently investigated.
Availability note (English)
Also available from the INIS Liaison Officer for France, see the INIS website for current contact and E-mail addressesAbstract (French)
Dans cette these, apres un bref rappel des connaissances sur le systeme hypothalamus-hypophyse, on discernera trois parties. Dans la premiere, on situera les hormones peptidiques etudiees dans leur contexte biologique. L'etude du marquage radioactif de peptides hormonaux constituera la deuxieme partie: elle comprendra, outre l'application de methodes de tritiation deja connues a des problemes particuliers, la description d'une nouvelle methode de marquage par le tritium ainsi que l'utilisation du carbone 11 pour l'etude cinetique de repartition d'une hormone. La troisieme partie consistera en l'etude physico-chimique d'une hormone hypothalamique. Souhaitant contribuer a l'etude de la regulation de la fonction gonadotrope hypophysaire, on a oriente ces travaux concernant chacune des trois parties citees, vers l'hormone luteinisante (LH) et son facteur hypothalamique de liberation (LRF). L'etude de ce dernier a souleve, et par suite nous a permis d'etudier, le probleme des peptides contenant du tryptophane.Files
54007484.pdf
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Additional details
Additional titles
- Original title (French)
- Mises au point de nouvelles methodes de marquage radioactif (3H et 11C) de l'hormone luteinisante (LH) et de son facteur de liberation (LRF). Etude des proprietes physico-chimiques du LRF par dichroisme circulaire et spectroscopie d'emission
Publishing Information
- Imprint Pagination
- 228 p.
- Report number
- FRNC-TH--589
INIS
- Country of Publication
- France
- Country of Input or Organization
- France
- INIS RN
- 54007484
- Subject category
- S38: RADIATION CHEMISTRY, RADIOCHEMISTRY AND NUCLEAR CHEMISTRY;
- Resource subtype / Literary indicator
- Thesis
- Descriptors DEI
- CARBON 11; EMISSION SPECTROSCOPY; LABELLING; LH-RH; RADIOCHEMISTRY; STRUCTURAL CHEMICAL ANALYSIS; TRITIUM
- Descriptors DEC
- BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BETA-PLUS DECAY RADIOISOTOPES; CARBON ISOTOPES; CHEMISTRY; EVEN-ODD NUCLEI; HORMONES; HYDROGEN ISOTOPES; ISOTOPES; LIBERINS; LIGHT NUCLEI; MINUTES LIVING RADIOISOTOPES; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; PEPTIDE HORMONES; PITUITARY HORMONES; PROTEINS; RADIOISOTOPES; SPECTROSCOPY; YEARS LIVING RADIOISOTOPES
Optional Information
- Notes
- This record replaces 07241915