Published July 15, 2013 | Version v1
Journal article Metadata-only

Laser-induced fusion of human embryonic stem cells with optical tweezers

Creators

  • 1. Department of Mechanical and Biomedical Engineering, City University of Hong Kong (Hong Kong)
  • 2. Department of Biology and Chemistry, City University of Hong Kong (Hong Kong)
  • 3. Stem Cell and Regenerative Medicine Consortium, and Departments of Medicine and Physiology, LKS Faculty of Medicine, University of Hong Kong (Hong Kong)
  • 4. Center of Cardiovascular Research, Mount Sinai School of Medicine, New York, New York 10029 (United States)

Description

We report a study on the laser-induced fusion of human embryonic stem cells (hESCs) at the single-cell level. Cells were manipulated by optical tweezers and fused under irradiation with pulsed UV laser at 355 nm. Successful fusion was indicated by green fluorescence protein transfer. The influence of laser pulse energy on the fusion efficiency was investigated. The fused products were viable as gauged by live cell staining. Successful fusion of hESCs with somatic cells was also demonstrated. The reported fusion outcome may facilitate studies of cell differentiation, maturation, and reprogramming

Additional details

Identifiers

DOI
10.1063/1.4813822;

Publishing Information

Journal Title
Applied Physics Letters
Journal Volume
103
Journal Issue
3
Journal Page Range
p. 033701-033701.4
ISSN
0003-6951
CODEN
APPLAB

INIS

Country of Publication
United States
Country of Input or Organization
International Atomic Energy Agency (IAEA)
INIS RN
44078629
Subject category
S71: CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS;
Descriptors DEI
BIOLOGICAL EFFECTS; BIOPHYSICS; CELL DIFFERENTIATION; EFFICIENCY; FLUORESCENCE; IRRADIATION; LASER RADIATION; PROTEINS; PULSES; RADIATION PRESSURE; STEM CELLS
Descriptors DEC
ANIMAL CELLS; ELECTROMAGNETIC RADIATION; EMISSION; LUMINESCENCE; ORGANIC COMPOUNDS; PHOTON EMISSION; PHYSICS; RADIATIONS; SOMATIC CELLS

Optional Information

Notes
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