Published February 23, 2000
| Version v1
Miscellaneous
Open
Rapid Detection Of Escherichia coli Enterohemorragic (EHEC) Bacteria by PCR (Polymerase Chain Reaction) methods
Creators
- 1. Center for Research and Development of Nuclear Tehniques, National Nuclear Energy Agency, Bandung (Indonesia)
Description
A polymerase Chain Reaction (PCR) assay for detect presence of enterohemmoragic Eschericha coli O157:H7 was carried out. DNA was extracted from bacterial cells with CTBA-phenol-chloroform and precipitated with isopropanol. To test sensitivity of PCR amplifies reaction, serial dilutions of E. coli DNA solution were prepared bwtween 1 mu g-1 ng/mu l. A single pair oligonucleotide primer SLTI-F and SLTI-R derived from shiga-like-toxin genes was used in amplification method. The results shows that 1 ng/mu l of E. coli DNA could be detected using the primers SLTI-F and SLTI-R with the position of 140 bp DNA fragment
Files
34043556.pdf
Files
(1.9 MB)
Name | Size | Download all |
---|---|---|
md5:47f6f8f088e68f81c433107e128d0495
|
1.9 MB | Preview Download |
Additional details
Additional titles
- Original title (Indonesian)
- Deteksi Cepat Bakteri Escherichia coli Enterohemoragik (EHEK) Dengan Metode PCR (polymerase Chain Reaction)
Publishing Information
- Publisher
- Centre for Research and Development of Isotopes and Radiation Technolgy, National Nuclear Energy Agency
- Imprint Place
- Jakarta (Indonesia)
- ISBN
- 979-95709-5-6
- Imprint Title
- Proceedings of a Scientific Meeting on Research and Development of Isotopes and Radiation Technology. 1999/2000
- Imprint Pagination
- 295 p.
- Journal Page Range
- p. 75-80
- Report number
- INIS-ID--023
Conference
- Title
- Conference on the role of Isotopes and Radiation Technology to Make Prosperous Society
- Original Conference Title
- Peranan Teknologi Isotop dan Radiasi untuk Mensejahterakan masyarakat
- Dates
- 23-24 Feb 2000
- Place
- Jakarta (Indonesia)
INIS
- Country of Publication
- Indonesia
- Country of Input or Organization
- Indonesia
- INIS RN
- 34043556
- Subject category
- RADIOLOGY AND NUCLEAR MEDICINE;
- Resource subtype / Literary indicator
- Conference
- Quality check status
- Yes
- Descriptors DEI
- CHEMICAL REACTIONS; DETECTION; ENZYME IMMUNOASSAY; ESCHERICHIA COLI; PATHOGENS; POLYMERASE CHAIN REACTION;
- Descriptors DEC
- BACTERIA; BIOASSAY; GENE AMPLIFICATION; IMMUNOASSAY; MICROORGANISMS;
Optional Information
- Lead record
- qyqv3-av376
- Notes
- 10 refs; 1 tabs.; 3 figs. Imprint:Risalah Pertemuan Ilmiah Penelitian Dan Pengembangan Teknologi Isotop Dan Radiasi. 1999/2000. Tema: Peranan Teknologi Isotop dan Radiasi untuk Mensejahterakan Masyarakat. Prosiding