Published February 23, 2000 | Version v1
Miscellaneous Open

Rapid Detection Of Escherichia coli Enterohemorragic (EHEC) Bacteria by PCR (Polymerase Chain Reaction) methods

  • 1. Center for Research and Development of Nuclear Tehniques, National Nuclear Energy Agency, Bandung (Indonesia)

Description

A polymerase Chain Reaction (PCR) assay for detect presence of enterohemmoragic Eschericha coli O157:H7 was carried out. DNA was extracted from bacterial cells with CTBA-phenol-chloroform and precipitated with isopropanol. To test sensitivity of PCR amplifies reaction, serial dilutions of E. coli DNA solution were prepared bwtween 1 mu g-1 ng/mu l. A single pair oligonucleotide primer SLTI-F and SLTI-R derived from shiga-like-toxin genes was used in amplification method. The results shows that 1 ng/mu l of E. coli DNA could be detected using the primers SLTI-F and SLTI-R with the position of 140 bp DNA fragment

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Additional details

Additional titles

Original title (Indonesian)
Deteksi Cepat Bakteri Escherichia coli Enterohemoragik (EHEK) Dengan Metode PCR (polymerase Chain Reaction)

Publishing Information

Publisher
Centre for Research and Development of Isotopes and Radiation Technolgy, National Nuclear Energy Agency
Imprint Place
Jakarta (Indonesia)
ISBN
979-95709-5-6
Imprint Title
Proceedings of a Scientific Meeting on Research and Development of Isotopes and Radiation Technology. 1999/2000
Imprint Pagination
295 p.
Journal Page Range
p. 75-80
Report number
INIS-ID--023

Conference

Title
Conference on the role of Isotopes and Radiation Technology to Make Prosperous Society
Original Conference Title
Peranan Teknologi Isotop dan Radiasi untuk Mensejahterakan masyarakat
Dates
23-24 Feb 2000
Place
Jakarta (Indonesia)

INIS

Country of Publication
Indonesia
Country of Input or Organization
Indonesia
INIS RN
34043556
Subject category
RADIOLOGY AND NUCLEAR MEDICINE;
Resource subtype / Literary indicator
Conference
Quality check status
Yes
Descriptors DEI
CHEMICAL REACTIONS; DETECTION; ENZYME IMMUNOASSAY; ESCHERICHIA COLI; PATHOGENS; POLYMERASE CHAIN REACTION;
Descriptors DEC
BACTERIA; BIOASSAY; GENE AMPLIFICATION; IMMUNOASSAY; MICROORGANISMS;

Optional Information

Lead record
qyqv3-av376
Notes
10 refs; 1 tabs.; 3 figs. Imprint:Risalah Pertemuan Ilmiah Penelitian Dan Pengembangan Teknologi Isotop Dan Radiasi. 1999/2000. Tema: Peranan Teknologi Isotop dan Radiasi untuk Mensejahterakan Masyarakat. Prosiding