Expression property and antitumor effect of pEgr-angiostatin induced by ionizing radiation
- 1. Jilin Univ., Changchun (China). MH Radiobiology Research Unit, School of Public Health
- 2. Henan Univ. (China). Medical School, Dept. of Cytobiology
- 3. Jilin Univ., Changchun (China). The Second Hospital, Dept. of Radiationtherapy
Description
Objective: To study on the expression and antitumor effect of pEgr-angiostatin induced with ionizing radiation. Methods: Mouse angiostatin cDNA was amplified with RT-PCR. The pEgr-angiostatin recombinant plasmid was constructed and was then transfected into B16 cells with liposome. The cells were irradiated and the expression of angiostatin mRNA in B16 cells was detected. Finally, the antitumor effect of pEgr-angiostatin was studied. Results: The sequence of mouse angiostatin cDNA was identical to reported. Egr-1 promoter and angiostatin cDNA was inserted correctly into expression vector. The expression property of pEgr-angiostatin was examined by ionizing radiation. The gene-therapy with pEgr-angiostatin showed remarkably antitumor effect. Conclusion: Mouse angiostatin cDNA had been cloned successfully in the study. The expression property and the antitumor effect of pEgr-angiostatin are induced by ionizing radiation. This result provides the basis for multi-gene-radiotherapy of tumor
Additional details
Publishing Information
- Journal Title
- Journal of Jilin University. Medicine Edition
- Journal Volume
- 29
- Journal Issue
- 5
- Journal Page Range
- p. 543-546
- ISSN
- 1671-587X
INIS
- Country of Publication
- China
- Country of Input or Organization
- China
- INIS RN
- 35089646
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Descriptors DEI
- GENES; INHIBITION; IONIZING RADIATIONS; MESSENGER-RNA; NEOPLASMS; RADIOINDUCTION; RADIOTHERAPY; RATS; X RADIATION
- Descriptors DEC
- ANIMALS; DISEASES; ELECTROMAGNETIC RADIATION; IONIZING RADIATIONS; MAMMALS; MEDICINE; NUCLEAR MEDICINE; NUCLEIC ACIDS; ORGANIC COMPOUNDS; RADIATIONS; RADIOLOGY; RNA; RODENTS; THERAPY; VERTEBRATES