A freezing technique applicable to transformation studies of C3H10T1/2 cells

The authors conclude that their freezing technique is applicable to studies of the dose-protraction effect of fission spectrum neutrons on neoplastic transformations, using the C3H10 T 1/2 cell mouse embryo fibroblast system, at least for storage of irradiated cells for up to 1 week. Results showed that the freezing process shifted the distribution of plating efficiencies towards somewhat lower values, but that the mean value for frozen cells was still within an acceptable region. (UK)