Clinical value of determination on serum IL-2, MMP-9, TK1 and sFas levels both before and after operation in patients with breast cancer

Objective: To discuss clinical value of determination of serum IL-2, MMP-9, TKl and sFas levels before and after operation in patients with breast cancer. Methods: Serum level of IL-2 was determined with RIA, serum levels of MMP-9 and sFas were determined with EIA. Serum level of TK1 was determined with CIS-1 immune blot enhanced chemiluminescence detection system. Results: Postoperative patients with breast cancer the serum level of IL-2 showed significantly increased than that in patients before operation (P < 0.01), before operation the serum level of IL-2 proved significantly lower than that in the controls and healthy woman (all P < 0.05). In postoperative patients group, the serum level of MMP-9 expressed significantly dropped than that of patients before operation (P < O.05), before operation the serum level of MMP-9 was significantly higher than that in the controls and healthy woman (all P < 0.05). In postoperative patients group, the serum level of TK1 appeared significantly dropped than preoperative (P < 0.05), before operation the serum level of MMP-9 presented significantly higher than that in controls and healthy woman (all P < 0.05). The changes of serum levels on sFas as well as in MMP-9. The results of correlative analysis showed, the serum level of TK1 with MMP-9 and sFas levels proved mutually positively correlated (r = 0.548, P < 0.01; r = 0.241, P < 0.05), while the serum level of TK1 with IL-2 level presented positivety correlation (r = -0. 221, P < 0.05). Conclusion: The changes of 4 serum indexes levels were related with the development of breast cancer, the determination might be helpful for pathogenesis and prediction. (authors)