Method for analysis of electrophoregrams with spectral resolution of fluorescence

The paper proposed model of the scanning laser device with high spectral resolution for detecting fluorescent proteins on electrophoregrams. Its design is based on the movable by micrometric screws platform, on which the holder of the optical fiber of the laser fluorescence spectrometer is fixed perpendicular to the plane under study. The device was developed to analyze the binding of tricarbocyanine dyes to blood plasma proteins using protein electrophoresis (sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing conditions, Laemmli method). A laser with a wavelength of 684 nm was used to excite fluorescence. The supply of exciting radiation to the sample under study and the fluorescence into the polychromator was carried out using an optical fiber. It is shown that the fluorescence spectra registration provides additional information for the identification of the fluorescent label and analyze its condition. On the example of a study using gel electrophoresis of solutions of bovine serum albumin stained with tricarbocyanine dyes, the possibility of detecting and identifying complexes of dyes with blood serum proteins has been shown. (authors)