Published September 15, 1981
| Version v1
Journal article
An NMR probe for the study of aerobic suspensions of cells and organelles
Description
The construction of an NMR probe and cell chamber with good mixing, pH buffering, and oxygenation characteristics, which can be used for relatively dilute cell and organelle suspension is described. The 31P NMR spectra of acceptable signal-to-noise ratios are obtained from approximately 200 mg (protein) of tissues, and kinetic studies of mitochondrial oxidative phosphorylation are demonstrated. Representative spectra from rabbit kidney cortical tubules and rabbit kidney cortical mitochondria are presented
Additional details
Publishing Information
- Journal Title
- Anal. Biochem.
- Journal Volume
- 116
- Journal Issue
- 2
- Series
- Anal. Biochem.
- Journal Page Range
- 450-455
- ISSN
- 0003-2697
INIS
- Country of Publication
- United States
- Country of Input or Organization
- United States
- INIS RN
- 14792179
- Subject category
- S62: RADIOLOGY AND NUCLEAR MEDICINE;
- Descriptors DEI
- AEROBIC CONDITIONS; ANIMAL CELLS; BIOCHEMICAL REACTION KINETICS; MITOCHONDRIA; NMR SPECTRA; PH VALUE; PHOSPHORUS 31; RABBITS; SUSPENSIONS; TUBULES
- Descriptors DEC
- ANIMALS; BODY; CELL CONSTITUENTS; DISPERSIONS; ISOTOPES; KIDNEYS; KINETICS; LIGHT NUCLEI; MAMMALS; NUCLEI; ODD-EVEN NUCLEI; ORGANOIDS; ORGANS; PHOSPHORUS ISOTOPES; REACTION KINETICS; SPECTRA; STABLE ISOTOPES; VERTEBRATES