Pathological changes in fish blood erythrocytes under the joint exposure to crude oil of high concentration and starvation

The paper analyzes the joint effect of high concentration of crude oil (500 mg/l) and starvation on the erythrocytes of Kura carp's blood. The material was taken at 5, 10, 14 days after exposure. Staining of blood smears was performed by the method of Romanovsky with the Gimsa stain. The study showed that the combined effect of high concentration of crude oil and starvation causes a number of the pathological changes in red blood cells of fish. Thus, in the course of the experiment in blood smears of fish were found the protrusions of membrane of cells, scalloped red blood cells, hypochromasia, the displacement of the nucleus, chromatinolysis, kariolysis. In addition, amitosis, nuclear-free red blood cells and red blood cells with two nuclei were observed in red blood cells. Also, this study showed an increase in the percentage of anisacytosis, poikilocytosis and destruction of red blood cells on individual fragments. (author)