Radiometric investigation of biosynthesis of certain macromolecules in cell cultures inoculated with intracellularly multiplied Yersinia pseudotuberculosis and Listeria monocytogenes

The study was conducted to investigate the changes in the biosynthesis of DNA, RNA and protein in epithelian cells from monkey kidney in the case of invasion by initial and passage Yersinia and Listeria strains, multiplied intracellularly. The Yersinia pseudotuberculosis strains IB 373 and IB 373-GP and Listeria monocytogenes strains 760 1,2a and 383-4b obtained by several passages through guinea pigs and their passage subculture were used. Inoculated cultures were labelled with 3H-thymidine, 3H uridine and 14C-valine. The results showed that in all cases 3H-thymidine and 3H-uridine inclusions into cell cultures, invaded with intracellularly multiplied Yersinia strains showed statistically higher values as compared to those from cell cultures invaded with the initial strain. 14C-valine inclusion in cells, invaded by passage strains, decreased reaching its lowest values in cell populations invaded by Yersiniae isolated during the 9th passage. In cell cultures, invaded by passage L. monocytogenes strains, a rise in the levels of 3H-thymidine and 3H-uridine inclusions was observed. Inclusion values of the precursors were higher in cell populations invaded by the monocytogenes 760-1,2a initial strain and by passage strains obtained from it. The 14C-valine inclusion varied in epithelial cells invaded by Listeriae of sero-type 1,2a and by those of sero type 4b. In the first group the highest inclusion values were recorded in epithelian cells invaded by Listeriae of the 1st passage and in the second in those invaded by Listerae isolated during the 3rd and 4th passages when their most active multiplication accured