Assessment of the response of human bladder cancer cell lines to radiation using colony formation assay and [3H] thymidine incorporation assay
- 1. Kyoto Univ. (Japan). Faculty of Medicine
Description
The effect of radiation on the human bladder cancer cell lines, KK47 and J82, was studied using colony formation assay and [3H] thymidine incorporation assay. The response to radiation was dose-related for both assays, but the curve for the colony formation assay was steeper than that for [3H] thymidine incorporation assay. However, the steepness of the curve for [3H] thymidine incorporation assay increased to be approximately the same as that for the colony formation assay as the incubation period increased. [3H] Thymidine incorporation assay can provide a rapid assessment of radiation sensitivity comparable to that obtained by the colony formation assay to select a radiosensitive bladder cancer patient for definitive or adjunctive radiotherapy. (author)
Additional details
Publishing Information
- Journal Title
- Hinyokika Kiyo
- Journal Volume
- 34
- Journal Issue
- 1
- Series
- Hinyokika Kiyo.
- Journal Page Range
- 32-36
- ISSN
- 0018-1994
- CODEN
- HIKYA
INIS
- Country of Publication
- Japan
- Country of Input or Organization
- Japan
- INIS RN
- 19067105
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Descriptors DEI
- BLADDER; CELL CULTURES; COLONY FORMATION; DOSE-RESPONSE RELATIONSHIPS; RADIATION DOSES; RADIOSENSITIVITY; SURVIVAL CURVES; THYMIDINE; TRITIUM; TUMOR CELLS; UPTAKE
- Descriptors DEC
- ANIMAL CELLS; AZINES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BODY; HETEROCYCLIC COMPOUNDS; HYDROGEN ISOTOPES; ISOTOPES; LIGHT NUCLEI; NUCLEI; NUCLEOSIDES; NUCLEOTIDES; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; PYRIMIDINES; RADIOISOTOPES; RIBOSIDES; URINARY TRACT; YEARS LIVING RADIOISOTOPES