Radioimmunodetection using anti-TPA iodine-125 in SD rats suffering from Walker's carcinosarcoma and ACI rats having carcinomas of the bladder
Description
A total of sixty-six rats were included in a study on radioimmunodetection. During these experiments, attempts were made on the basis of five different methods were used in an attempt to detect the injected antibodies. These were scintigraphy, flow measurements, activity determinations in both the intracellular and extracellular spaces, autoradiogrammes and PaP staining. The use of a range of various methods of detection turned out to be very advantageous, as the successful visualisation of the tumours in the scintigrammes and their marked activity concentrations of might have been interpreted as an indication of specific binding of anti-TPA to the tumour grafts. The absence of any such specific reactions was, however, quite obvious from the fact that TPA could not be detected in the tumour models used for the same purpose. (orig./MG)
Availability note (English)
Available from the library of Bonn Univ. (Germany).Abstract (German)
Insgesamt wurden an sechsundsechzig Ratten Versuche zur Radioimmunodetection durchgefuehrt. Dabei versuchte man die injizierten Antikoerper mit fuenf verschiedenen Methoden nachzuweisen: Szintigraphisch, durch Flowmessungen, durch Aktivitaetsbestimmungen im Intra- und Extrazellulaerraum, durch Autoradiographie und PaP-Faerbung. Diese Vielzahl an Nachweismethoden hat sich als guenstig erwiesen, da man aufgrund der gelungenen szintigraphischen Tumordarstellungen und der starken Aktivitaetsanreicherung im Tumor zu dem Schluss haette kommen koennen, dass sich anti-TPA spezifisch an die Tumortransplantate anlagert. Dass diese Anlagerung aber nicht spezifisch sein kann, ergibt sich daraus, dass TPA in den verwendeten Tumormodellen nicht nachgewiesen werden konnte. (orig./MG)Additional details
Additional titles
- Original title (German)
- Radioimmunodetection mit anti-TPA
Publishing Information
- Imprint Pagination
- 135 p.
INIS
- Country of Publication
- Germany
- Country of Input or Organization
- Germany
- INIS RN
- 23019073
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; S62: RADIOLOGY AND NUCLEAR MEDICINE;
- Resource subtype / Literary indicator
- Thesis, Non-conventional Literature
- Descriptors DEI
- ACCURACY; ANTIBODIES; AUTORADIOGRAPHY; CARCINOMAS; CYTOLOGICAL TECHNIQUES; DIAGNOSTIC TECHNIQUES; EXPERIMENTAL NEOPLASMS; IODINE 125; RADIOIMMUNOASSAY; RATS; SARCOMAS; SCINTISCANNING; STAINS
- Descriptors DEC
- ANIMALS; BETA DECAY RADIOISOTOPES; COUNTING TECHNIQUES; DAYS LIVING RADIOISOTOPES; DISEASES; ELECTRON CAPTURE RADIOISOTOPES; IMMUNOASSAY; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTO; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MAMMALS; NEOPLASMS; NUCLEI; ODD-EVEN NUCLEI; RADIOISOTOPE SCANNING; RADIOISOTOPES; RODENTS; TRACER TECHNIQUES; VERTEBRATES