Hydrogen sulfide ameliorated lipopolysaccharide-induced acute lung injury by inhibiting autophagy through PI3K/Akt/mTOR pathway in mice

Highlights: • Autophagy activation is an important pathogenesis of LPS-induced ALI in mouse. • H₂S can inhibit the activation of autophagy in ALI induced by LPS. • H₂S and 3-MA ameliorated LPS-induced ALI both in vivo and in vitro. • H₂S inhibit the activation of autophagy through PI3K/Akt/mTOR pathway. Recent studies reported that hydrogen sulfide (H₂S) is an effective agent for the prevention and treatment of acute lung injury (ALI). But the underlying mechanisms have not been understood clearly. In this study, we explored the possible mechanism from the perspective of autophagy regulation.