Vasoactive intestinal peptide and electrical activity influence neuronal survival
Description
Blockage of electrical activity in dissociated spinal cord cultures results in a significant loss of neurons during a critical period in development. Decreases in neuronal cell numbers and 125I-labeled tetanus toxin fixation produced by electrical blockage with tetrodotoxin (TTX) were prevented by addition of vasoactive intestinal peptide (VIP) to the nutrient medium. The most effective concentration of VIP was 0.1 nM. At higher concentrations, the survival-enhancing effect of VIP on TTX-treated cultures was attenuated. Addition of the peptide alone had no significant effect on neuronal cell counts or tetanus toxin fixation. With the same experimental conditions, two closely related peptides, PHI-27 (peptide, histidyl-isoleucine amide) and secretin, were found not to increase the number of neurons in TTX-treated cultures. Interference with VIP action by VIP antiserum resulted in neuronal losses that were not significantly different from those observed after TTX treatment. These data indicate that under conditions of electrical blockade a neurotrophic action of VIP on neuronal survival can be demonstrated
Additional details
Publishing Information
- Journal Title
- Proc. Natl. Acad. Sci. U.S.A
- Journal Volume
- 83
- Journal Issue
- 4
- Series
- Proc. Natl. Acad. Sci. U.S.A.
- Journal Page Range
- 1159-1162
- ISSN
- 0027-8424
- CODEN
- PNASA
INIS
- Country of Publication
- United States
- Country of Input or Organization
- United States
- INIS RN
- 18089700
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- BIOCHEMISTRY; BIOELECTRICITY; CARDIOVASCULAR AGENTS; CELL CULTURES; CELL DIFFERENTIATION; CELL KILLING; INTESTINES; IODINE 125; NERVE CELLS; PEPTIDES; SPINAL CORD; TETANUS; TOXINS; UPTAKE
- Descriptors DEC
- ANIMAL CELLS; ANTIGENS; BACTERIAL DISEASES; BETA DECAY RADIOISOTOPES; BODY; CENTRAL NERVOUS SYSTEM; CHEMISTRY; DAYS LIVING RADIOISOTOPES; DIGESTIVE SYSTEM; DISEASES; DRUGS; ELECTRICITY; ELECTRON CAPTURE RADIOISOTOPES; GASTROINTESTINAL TRACT; INFECTIOUS DISEASES; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTO; IODINE ISOTOPES; ISOTOPES; NERVOUS SYSTEM; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANS; PROTEINS; RADIOISOTOPES; SOMATIC CELLS