Published November 1990 | Version v1
Journal article

Investigation of the repair of single-strand breaks in human DNA using alkaline gel electrophoresis

  • 1. University Clinics, Kantonsspital, Basel (Switzerland)

Description

Unstimulated lymphocytes from eight healthy persons were exposed to 10-, 30-, and 100-Gy doses of 60Co gamma radiation. The repair of damaged DNA was measured by (1) alkaline gel electrophoresis (extracted DNA loaded on 0.25% agarose gel, run at 1 V/cm for 39-44 h) at 0, 1, and 2 h after exposure and (2) incorporation of [3H]thymidine into unstimulated lymphocytes in the presence of 2 mM hydroxyurea 1 and 2 h after exposure. Both methods--alkaline gel electrophoresis and thymidine incorporation--showed that repair was completed within 2 h

Additional details

Publishing Information

Journal Title
Radiation Research
Journal Volume
124
Journal Issue
2
Series
Radiat. Res.
Journal Page Range
137-140
ISSN
0033-7587
CODEN
RAREA