Published March 2005
| Version v1
Journal article
Development of a liquid phase immunoassay by time-dependent measurements of the transient magneto-optical birefringence using functionalized magnetic nanoparticles
- 1. Institut fuer Pharmazie, Ernst-Moritz-Arndt-Universitaet, Friedrich-Ludwig-Jahn-Str. 17, D-17487 Greifswald (Germany)
- 2. Institut fuer Festkoerperphysik, Friedrich-Schiller-Universitaet, Helmholtzweg 5, D-07743 Jena (Germany)
Description
The transient magneto-optical birefringence was investigated as a new liquid phase immunoassay technique. Specifically binding magnetic probes using monoclonal as well as polyclonal antibodies were investigated. The detection limit for optimized magnetic nanoparticles was determined. Binding experiments of polyclonal probes with their specific antigen yielded increased particle sizes as determined from magneto-optical relaxation curves. Aggregation of magnetic probes due to cross-linking caused gradually decreased signal intensity
Additional details
Identifiers
- DOI
- 10.1016/j.jmmm.2004.11.135;
- PII
- S0304-8853(04)01373-3;
Publishing Information
- Journal Title
- Journal of Magnetism and Magnetic Materials
- Journal Volume
- 289
- Journal Page Range
- p. 480-483
- ISSN
- 0304-8853
- CODEN
- JMMMDC
Conference
- Title
- 10. international conference on magnetic fluids
- Acronym
- ICMF10
- Dates
- 2-6 Aug 2004
- Place
- Sao Paulo (Brazil)
INIS
- Country of Publication
- Netherlands
- Country of Input or Organization
- International Atomic Energy Agency (IAEA)
- INIS RN
- 37024806
- Subject category
- S36: MATERIALS SCIENCE;
- Resource subtype / Literary indicator
- Conference
- Descriptors DEI
- ANTIGENS; BIREFRINGENCE; IMMUNOASSAY; LIQUIDS; MAGNETIC MATERIALS; MAGNETIC PROBES; NANOSTRUCTURES; PARTICLE SIZE; PARTICLES; RELAXATION; SENSITIVITY
- Descriptors DEC
- BIOASSAY; FLUIDS; MATERIALS; PROBES; REFRACTION; SIZE
Optional Information
- Copyright
- Copyright (c) 2004 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.