Published March 24, 2005
| Version v1
Journal article
Expression, purification and crystallization of a human protein SH3BGRL at atomic resolution
- 1. Graduate School of the Chinese Academy of Sciences, Beijing 100039 (China)
- 2. Center for Structural and Molecular Biology, Institute of Biophysics, Chinese Academy of Science, Beijing 100101 (China)
- 3. Shanghai Institute of Hematology, Rui Jin Hospital, Shanghai Second Medical University, Shanghai 200025 (China)
Description
The protein SH3BGRL, containing both SH3-binding and Homer EVH1-binding motifs, has been crystallized using the hanging-drop vapour-diffusion method. The protein SH3BGRL, containing both SH3-binding and Homer EVH1-binding motifs, has been crystallized using the hanging-drop vapour-diffusion method. The crystals diffract to 0.88 Å resolution and belong to space group P212121, with unit-cell parameters a = 28.8886, b = 34.9676, c = 98.0016 Å. Preliminary analysis indicates that the asymmetric unit contains one molecule and has a solvent content of about 34%
Availability note (English)
Available from http://dx.doi.org/10.1107/S174430910500730X; Available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1952435Additional details
Identifiers
- URL
- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1952435;
- DOI
- 10.1107/S174430910500730X;
- PII
- S174430910500730X;
Publishing Information
- Journal Title
- Acta Crystallographica. Section F
- Journal Volume
- 61
- Journal Issue
- Pt 4
- Journal Page Range
- p. 384-386
- ISSN
- 1744-3091
- CODEN
- ACSFCL
INIS
- Country of Publication
- United Kingdom
- Country of Input or Organization
- International Atomic Energy Agency (IAEA)
- INIS RN
- 46069246
- Subject category
- S75: CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY;
- Descriptors DEI
- CRYSTALLIZATION; CRYSTALS; DIFFUSION; MOLECULES; PROTEINS; RESOLUTION; SOLVENTS; SPACE GROUPS
- Descriptors DEC
- ORGANIC COMPOUNDS; PHASE TRANSFORMATIONS; SYMMETRY GROUPS
Optional Information
- Copyright
- Copyright (c) International Union of Crystallography 2005
- Notes
- PMCID: PMC1952435; PMID: 16511048; PUBLISHER-ID: pu5067; OAI: oai:pubmedcentral.nih.gov:1952435