Electrochemical detection of insulin

This work deals with electrochemical determination of insulin on gold and carbon screen printed electrodes using cyclic voltammetry method. Mentioned electrodes have significantly smaller size compared to classic electrodes, achieving miniaturization of the system. The aim of the work was to find optimal conditions for insulin determination, while impact of pH and scan rate was observed. Based on the execution of measurements, correlation coefficient was determined, increase of which was obtained by modifying the electrodes with nanoparticles. In case of carbon screen printed electrode correlation coefficient was increased form -0,18 (unmodified electrode) to 0,87 (carbon electrode modified with CNTs and NiNPs). The active areas of the electrodes was also determined by the method of cyclic voltammetry. Modification with nanoparticles achieved a rapid increase of the active surfaces compared to the unmodified electrodes. In case of modification of gold screen printed electrode with the combination of CNTs and AuNPs, an increase of active area of the electrode was achieved by up to 183% (authors)