Published October 1995 | Version v1
Journal article Metadata-only

Imaging studies of breast cancer xenografts. Monoclonal anti-mucin antibodies BM-7 and 12H12 labeled with 99mTC

Creators

  • 1. Onkologisches Labor, Universitaetsfrauenklinik, Heidelberg (Germany)
  • 2. Radiologische Diagnostik und Therapie, Deutsches Krebsforschungszentrum, Heidelberg (Germany)

Description

The present study was undertaken to evaluate the correlation of the favourable in vitro characteristics of the anit-mucin Mabs 12H12 and BM-7 with high tumor accumulation in vivo. They were labeled with 99mTc; their biodistribution in nude mice bearing mammary tumor xenograft AR was examined and immunoscintigraphy was performed after 24 h. 99mTc-labeling of the Mabs 12H12 and BM-7 led to tumor uptakes of 20.7% and 8.8% ID/g, respectively, after 48 h. Tumor-to-muscle ratios were 31 (12H12) and 18 (BM-7). Tumor xenografts were clearly visualized in immunoscintigrams. Combination of Mab 12H12 and 99mTc provides high tumor-to-tissue ratios shortly after administration. (orig.)

Abstract (German)

Gegenstand dieser Arbeit waren tierexperimentelle Untersuchungen zur Biodistribution und Immunszintigraphie der Anti-Muzin-MoAbs 12H12 und BM-7 in thymusaplastischen Nacktmaeusen mit xenotransplantierten humanen Mammakarzinomen. Die MoAb waren mit 99mTc markiert; ihre Biodistribution wurde im Tiermodell 24 und 48 h nach i. v. Injektion untersucht. Die 99mTc-Markierungen der MoAbs 12H12 und BM-7 fuehrten zu einer Tumoranreicherung von 20,7% bzw. 8,8% ID/g und zu Tumor/Muskel-Quotienten von 31 bzw. 18 nach 48 h. Immunszintigraphisch liess sich mit beiden MoAbs nach 24 h das Mammakarzinom AR gut darstellen. Die beiden MoAbs erfahren im Tiermodell eine schnelle Tumoraufnahme und rasche Blut-Clearance. (orig.)

Additional details

Additional titles

Original title (German)
Immunszintigraphie xenotransplantierter Mammakarzinome.

Publishing Information

Journal Title
Nuklearmedizin
Journal Volume
34
Journal Issue
5
Journal Page Range
p. 197-202.
ISSN
0029-5566
CODEN
NUKLDV

INIS

Country of Publication
Germany
Country of Input or Organization
Germany
INIS RN
27010781
Subject category
S60: APPLIED LIFE SCIENCES; S62: RADIOLOGY AND NUCLEAR MEDICINE;
Descriptors DEI
BLOOD-PLASMA CLEARANCE; CARCINOMAS; EXPERIMENTAL NEOPLASMS; INTRAVENOUS INJECTION; MAMMARY GLANDS; MICE; MONOCLONAL ANTIBODIES; RADIOIMMUNOSCINTIGRAPHY; RADIOPHARMACEUTICALS; TECHNETIUM 99; TISSUE DISTRIBUTION; TRANSPLANTS; UPTAKE
Descriptors DEC
ANIMALS; ANTIBODIES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BODY; CLEARANCE; COUNTING TECHNIQUES; DIAGNOSTIC TECHNIQUES; DISEASES; DISTRIBUTION; DRUGS; GLANDS; HOURS LIVING RADIOISOTOPES; INJECTION; INTAKE; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; ISOMERIC TRANSITION ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; LABELLED COMPOUNDS; MAMMALS; MATERIALS; NEOPLASMS; NUCLEI; ODD-EVEN NUCLEI; ORGANS; RADIOACTIVE MATERIALS; RADIOASSAY; RADIOIMMUNODETECTION; RADIOISOTOPE SCANNING; RADIOISOTOPES; RODENTS; SCINTISCANNING; TECHNETIUM ISOTOPES; TRACER TECHNIQUES; VERTEBRATES; YEARS LIVING RADIOISOTOPES