Published 1984 | Version v1
Journal article Metadata-only

Solid-phase radioimmunoassay for the detection of antibodies to collagens

Creators

  • 1. Institut Pasteur de Lyon, 69 (France)

Description

A solid-phase radioimmunoassay using 125I-protein A is described for the detection of antibodies to collagens of different types. The optimal conditions for the adsorption of collagen onto polystyrene microplates, then the incubations with the antiserum and finally with the 125I-protein A have been evaluated. The technique was applied successfully to antisera raised in rabbit, goat, guinea pig and mouse against human type I, II, III, IV, V and bovine type I, II, 1α2α3α, X1-X7 collagens

Abstract (French)

Nous presentons une technique radioimmunologique a la proteine A [125I] sur microplaque de polystyrene permettant le dosage des anticorps anti-collagenes de differents types. Les conditions optimales pour l'adsorption du collagene et l'incubation de l'immunserum puis de la proteine A [125I] ont ete determinees. La technique a ete appliquee avec succes au dosage d'anticorps anti-collagenes humains de types I, II, III, IV, V, et bovins de types I, II, X1-X7, 1α2α3α obtenus chez le Lapin, la Chevre, le Cobaye et la Souris

Additional details

Additional titles

Original title (French)
Dosage des anticorps anti-collagenes par technique radioimmunologique en phase solide

Publishing Information

Journal Title
C. R. Seances Soc. Biol. Fil.
Journal Volume
178
Journal Issue
2
Series
C. R. Seances Soc. Biol. Fil.
Journal Page Range
160-170
ISSN
0037-9026

INIS

Country of Publication
France
Country of Input or Organization
France
INIS RN
15067649
Subject category
S62: RADIOLOGY AND NUCLEAR MEDICINE;
Descriptors DEI
ANTIBODIES; COLLAGEN; IMMUNE SERUMS; IODINE 125; LABELLED COMPOUNDS; PROTEINS; RADIOIMMUNOASSAY
Descriptors DEC
BETA DECAY RADIOISOTOPES; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; RADIOISOTOPES; SCLEROPROTEINS; TRACER TECHNIQUES