A preliminary study on ¹³C enrichment of in cunninghamia lanceolata using pulse labeling technique

A pot experiment was conducted, by ¹³C pulse labeling technique, to characterize the enrichment of ¹³C in C. lanceolata plant and analyze the allocation of photosynthesis products among Chinese fir organs. The study aimed to provide a reference which about isotope labeling of woody plants and the allocation of photosynthesis carbon among plants results showed that the values of δ¹³C in needle and stem of unlabeled C. lanceolata decreased with time, and the values in root decreased at the beginning and then increased. The values of δ¹³C in different organs of labeled C. lanceolata initially increased and then decreased after the summit. The values of Atom% ¹³C of photosynthetic carbon allocation were different in C. lanceolata organs, with the largest value of Atom% ¹³C in current-needle, the smallest in 1-year-old needle, and the values in stem and root in the middle. After ¹³C pulse labeling, the stable carbon isotope ratios (δ ¹³C values) in needle, stem and root increased from -25.185‰, -24.689‰, -25.326‰ to 116.737‰, 106.800‰, 124.080‰, respectively. The above results indicated that plant materials which enriched ¹³C could be obtained with ¹³C pulse labeling technique, and could be useful for the study of turnover dynamics of soil carbon components. (authors)