The electrophilic lodi nation with 125 I/ 131 I of gamma globulin: Comparison between a solid-phase oxidizing agent (Iodogen), chloramine-T, iodine mono chloride and N-Bromo succinimide
Creators
- 1. Labelled Compounds Dept., Hot Laboratories Centre, Atomic Energy Authority. P. Code 13759, Cairo, (Egypt)
- 2. Faculty of Science-Zagazig University, (Egypt)
Description
A new available oxidizing agent, 1, 3, 4, 6-tetra chloro-3α, diphenyl glycoluril(iodogen) was compared with chloramine-T (Ch-T), Iodine-mono chloride (I Cl) and N-Bromo succinimide (NBS) in the radio-iodination of gamma-globulin (γ-G) with 'no-carrier-added' (nca) Na 131 I (T 1/2=8 d). In Phosphate and acetate buffer solution, the optimum reaction conditions with respect to PH, concentration of oxidizing agent, reaction time and concentration of γ-G were determined. The optimum conditions which were found require 100μ l of buffer (pH 7.4, 0.025 M), 500μg γ-G (0.003 μmol), (10-40) μg of oxidizing agent and the desired amount of carrier-free radioiodine. Highest radiochemical yield (>85-90%) were obtained at pH 7.4. Separation and identification of the labelled products were achieved by means of high performance liquid chromatography (HPLC) and thin layer chromatography (TLC). 8 figs., 1 tab
Additional details
Publishing Information
- Journal Title
- Arab Journal of Nuclear Sciences and Applications
- Journal Volume
- 30
- Journal Issue
- 1
- Journal Page Range
- p. 131.
- CODEN
- AJNADV
INIS
- Country of Publication
- Egypt
- Country of Input or Organization
- Egypt
- INIS RN
- 28066842
- Subject category
- S38: RADIATION CHEMISTRY, RADIOCHEMISTRY AND NUCLEAR CHEMISTRY;
- Resource subtype / Literary indicator
- Numerical Data
- Descriptors DEI
- CHLORAMINES; CHROMATOGRAPHY; EXPERIMENTAL DATA; GAMMA RADIATION; GLOBULINS; IODINATION; IODINE CHLORIDES; IODINE 125; IODINE 131; LABELLED COMPOUNDS; OXIDIZERS; PH VALUE
- Descriptors DEC
- AMINES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CHEMICAL REACTIONS; CHLORIDES; CHLORINE COMPOUNDS; DATA; DAYS LIVING RADIOISOTOPES; ELECTROMAGNETIC RADIATION; ELECTRON CAPTURE RADIOISOTOPES; HALIDES; HALOGEN COMPOUNDS; HALOGENATION; INFORMATION; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; IODINE COMPOUNDS; IODINE ISOTOPES; IONIZING RADIATIONS; ISOTOPES; NUCLEI; NUMERICAL DATA; ODD-EVEN NUCLEI; ORGANIC CHLORINE COMPOUNDS; ORGANIC COMPOUNDS; ORGANIC HALOGEN COMPOUNDS; PROTEINS; RADIATIONS; RADIOISOTOPES; SEPARATION PROCESSES