The protective effect of 2, 2-dimethylthiazolidine on human peripheral blood lymphocytes in radiation-induced genetic damage

Objective: To investigate the protective effect of 2, 2-dimethylthiazolidine (KFL) on human peripheral blood lymphocytes in radiation-induced genetic damage. Methods: Peripheral blood samples were collected from healthy volunteers and divided into normal control group, irradiation group and KFL protective groups with four different concentrations (0.2, 0.5, 1, 2 mmol/L). KFL was administrated 30 min before 2 Gy irradiation. Chromosome aberration analysis, micronucleus test and single cell gel electrophoresis were used to observe the protective effects of KFL on the radiation-induced genetic damage in human peripheral blood lymphocytes. Results: Compared with the irradiation group, the four KFL protection groups showed significant reduction in the chromosomal aberration frequencies (t = 5.34-25.48, P < 0.05), the micronucleus cell frequencies (t = 5.18-29.44, P < 0.05), the micronucleus frequencies (t = 4.67-12.04, P < 0.05), the tailleghth (t = 16.18-19.64, P < 0.05), the tailmoment (t = 11.79-13.01, P < 0.05), the olivetailmoment (t = 12.44-14.88, P < 0.05) and the tail DNA% of comet cells (t = 12.05-17.30, P < 0.05). The olivetailmoment of the two high concentrations in protective groups(1, 2 mmol/L) were significantly decreased compared with the other two KFL groups(0.2, 0.5 mmol/L), respectively (t = 5.67-16.56, P < 0.05). Compared with other KFL groups, the micronucleus frequencies and micronucleus cell frequencies of the 2 mmol/L KFL group was significantly decreased (t = 4.23-5.57, P < 0.05). Conclusions: KFL may have protective effect on human peripheral blood lymphocytes in the genetic damage induced by radiation. (authors)