Development of a new radio-immunologic proof for fibrinopeptide A

Because of the cross-reaction of antiserum with fibrinogen and other proteins, these proteins have to be separated from the plasma before the radio-immuno-assay (RIA) is carried out. Here a simplified and also much faster separation technique is introduced. A more efficient procedure is obtained, due to the RIA realization on micro titre plates. By separating the bound tracer from the free one by means of the double-antibody method, even more precise results could be achieved. In patients suffering from phlebothrombosis, infarction or hepatocirrhosis, the FpA concentrations were measured by applying the described simplified and rationalized RIA. The proposed RIA appears to be appropriate for routine examinations. (MG)