Classical & molecular cytogenetics as a tool for clinical investigation of genetic/acquired abnormalities

In this study, a comparative evaluation of classical and molecular cytogenetics is made with special emphasis on advancement of Fluorescence in-situ hybridization (FISH) based techniques. Cytogenetic investigation of a rare genetic abnormality, Wiskott Aldrich Syndrome (WAS), was carried out in a male child. Results indicated that the subject was prone to genetic instability when challenged with radiation stress. Another case of male sterility was cytogenetically analyzed, wherein the abnormality detected was the presence of two spots corresponding to sequences of Y-chromosome and this may contribute to the onset of infertility in the individual. (author)