Lethal effect of pyrimidine dimers induced at 365-nm in strains of E. coli differing in repair capability
Creators
- 1. Argonne National Lab., Ill. (USA)
- 2. Rio de Janeiro Univ. (Brazil). Instituto de Biofisica
Description
Photoreactivation (PR) after 365-nm inactivation was measured in four strains of Escherichia coli differing in repair capability. Photoreactivation was observed in the recA strains K12 AB2480 and K12 AB2463 indicating a significant role of pyrimidine dimers in the lethal action of 365-nm radiation in these strains. Significant PR was not observed in the uvrA strain, K12 AB1886, or in the repair proficient strain, K12 AB1157, after 365-nm inactivation. Biological evidence indicated that stationary phase cells had not lost the capacity for photo-enzymatic repair after fluences of 365-nm radiation of 2 x 106 J/m-2 or less. It is proposed that pyrimidine dimers, although induced, are not significant 365-nm lethal lesions in uvrA and wild-type strains because of their efficient dark repair
Additional details
Identifiers
Publishing Information
- Journal Title
- Mutation Research
- Journal Volume
- 37
- Journal Issue
- 2-3
- Series
- Mutat. Res.
- Journal Page Range
- 163-172
- ISSN
- 0027-5107
INIS
- Country of Publication
- Netherlands
- Country of Input or Organization
- Netherlands
- INIS RN
- 8298918
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Descriptors DEI
- DIMERS; ENZYMES; ESCHERICHIA COLI; LETHAL IRRADIATION; NEAR ULTRAVIOLET RADIATION; PHOTOREACTIVATION; PYRIMIDINES; SURVIVAL CURVES
- Descriptors DEC
- AZINES; BACTERIA; BIOLOGICAL RECOVERY; BIOLOGICAL REPAIR; ELECTROMAGNETIC RADIATION; HETEROCYCLIC COMPOUNDS; IRRADIATION; MICROORGANISMS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; RADIATIONS; ULTRAVIOLET RADIATION
Optional Information
- Notes
- Updated automatically by Metadata and Full-Text Enrichment Agent