Published 1976 | Version v1
Book Metadata-only

Insulin, ch. 17

Creators

  • 1. Mount Sinai School of Medicine, New York (USA)
  • 2. Veterans Administration Hospital, Bronx, N.Y. (USA)

Description

The radioimmunoassay of insulin is described. Iodination with 125I is performed by the chloramine-T method and the iodination mixture is purified by starch gel electrophoresis, sephadex G-50 gel filtration or by adsorption to and subsequent elution from a cellulose column. The requirements for the incubation mixtures are described in detail and a sample protocol is given. A separation of bound and free-labelled hormones is performed by adsorption, using talc or charcoal

Additional details

Publishing Information

Publisher
North-Holland.
Imprint Place
Amsterdam, Netherlands
ISBN
0 7204 0630 7
Imprint Title
Methods in radioimmunoassay of peptide hormones
Imprint Pagination
p. 168-174.

INIS

Country of Publication
Netherlands
Country of Input or Organization
Netherlands
INIS RN
8301324
Subject category
S60: APPLIED LIFE SCIENCES;
Descriptors DEI
ADSORPTION; AUTORADIOGRAPHY; CHROMATOGRAPHY; ELECTROPHORESIS; IMMUNE SERUMS; INCUBATION; INSULIN; IODINE 125; LABELLING; ORGANIC IODINE COMPOUNDS; RADIOCHROMATOGRAPHY
Descriptors DEC
BETA DECAY RADIOISOTOPES; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; HORMONES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPES; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANIC HALOGEN COMPOUNDS; PEPTIDE HORMONES; RADIOISOTOPES; SEPARATION PROCESSES

Optional Information

Notes
Imprint:Reprinted from Methods in Investigative and Diagnostic Endocrinology, vols. 2A and 2B, 1973, North-Holland.