Published September 2019
| Version v1
Journal article
Electrochemiluminescent competitive immunoassay for zearalenone based on the use of a mimotope peptide, Ru(II)(bpy)3-loaded NiFe2O4 nanotubes and TiO2 mesocrystals
- 1. College of Chemistry and Material & College of Physics and Energy Fujian Normal University (China)
- 2. Fujian Provincial Maternity and Children's Hospital (China)
- 3. Fuzhou University, Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, and Department of Chemistry (China)
Description
An ultrasensitive competitive-type electrochemiluminescence immunoassay for the mycotoxin zearalenone is described. The method is based on the use of (a) a mimotope peptide that was selected from a phage displayed peptide library and used to substitute ZEN for designing the competitive assay; (b) NiFe2O4 nanotubes with large specific surface area loaded with the ECL probe Ru(bpy)32+; and (c) poly(vinylpyrrolidone) (PVP)-assisted synthesis of TiO2 mesocrystals that acts as the sensing platform and support for antibody immobilization. Under the optimized conditions and at an ECL working potential of 1.1 V, a linear response is found for ZEN in the 0.1 to 1.0 × 10−5 ng·mL−1 concentration range with a detection limit as low as 3.3 fg·mL−1.
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Additional details
Identifiers
Publishing Information
- Journal Title
- Mikrochimica Acta
- Journal Volume
- 186
- Journal Issue
- 9
- Journal Page Range
- p. 1-8
- ISSN
- 0026-3672
- CODEN
- MIACAQ
INIS
- Country of Publication
- Austria
- Country of Input or Organization
- Austria
- INIS RN
- 52067393
- Subject category
- S77: NANOSCIENCE AND NANOTECHNOLOGY; S37: INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY;
- Descriptors DEI
- BIPYRIDINES; CHEMILUMINESCENCE; FERRITES; IMMUNOASSAY; MYCOTOXINS; NANOCHEMISTRY; NANOTUBES; NICKEL COMPOUNDS; PEPTIDES; PVP; RUTHENIUM COMPLEXES; TITANIUM OXIDES
- Descriptors DEC
- AMIDES; ANTIGENS; AZINES; AZOLES; BIOASSAY; BLOOD PLASMA; BLOOD SUBSTITUTES; CHALCOGENIDES; CHEMISTRY; COMPLEXES; DRUGS; EMISSION; FERRIMAGNETIC MATERIALS; HAZARDOUS MATERIALS; HEMATOLOGIC AGENTS; HETEROCYCLIC COMPOUNDS; IRON COMPOUNDS; LACTAMS; LUMINESCENCE; MAGNETIC MATERIALS; MATERIALS; NANOSTRUCTURES; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANIC POLYMERS; OXYGEN COMPOUNDS; PHOTON EMISSION; POLYMERS; POLYVINYLS; PROTEINS; PYRIDINES; PYRROLES; PYRROLIDONES; TOXIC MATERIALS; TOXINS; TRANSITION ELEMENT COMPLEXES; TRANSITION ELEMENT COMPOUNDS
Optional Information
- Copyright
- Copyright (c) 2019 Springer-Verlag GmbH Austria, part of Springer Nature