Published July 1980 | Version v1
Journal article Metadata-only

Radioimmunoassay for rhodopsin

Creators

  • 1. Case Western Reserve Univ., Cleveland, OH (USA). School of Medicine

Description

A radioimmunoassay for rhodopsin was devised using [125I]rhodopsin and antibody to purified bovine rhodopsin in a double antibody procedure that can quantify the concentration of this visual pigment when present in picomolar amounts. The method demonstrated a high degree of sensitivity, reproducibility, and specificity, and is applicable to the determination of the rhodopsin concentration in crude extracts of tissue as well as in more purified preparations. (author)

Additional details

Publishing Information

Journal Title
Exp. Eye Res.
Journal Volume
31
Journal Issue
1
Series
Exp. Eye Res.
Journal Page Range
1-8
ISSN
0014-4835

INIS

Country of Publication
United Kingdom
Country of Input or Organization
United Kingdom
INIS RN
12585145
Subject category
S60: APPLIED LIFE SCIENCES;
Descriptors DEI
ANTIGEN-ANTIBODY REACTIONS; BLOOD SERUM; GLYCOPROTEINS; IODINATION; IODINE 125; LABELLED COMPOUNDS; LABELLING; PIGMENTS; RABBITS; RADIOIMMUNOASSAY; RETINA; SENSITIVITY; SPECIFICITY
Descriptors DEC
ANIMALS; BETA DECAY RADIOISOTOPES; BODY; CARBOHYDRATES; CHEMICAL REACTIONS; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; EYES; HALOGENATION; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MAMMALS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANS; PROTEINS; RADIOISOTOPES; SACCHARIDES; SENSE ORGANS; TRACER TECHNIQUES; VERTEBRATES