Published 1988 | Version v1
Miscellaneous Metadata-only

'In vitro' determination of the rate of 32P uptake by erythrocytes

Creators

  • 1. Centro de Energia Nuclear na Agricultura, Piracicaba (Brazil)

Description

An ''in vitro'' methodology based on 32p uptake by erythrocytes was established as a potencial method for a phosphorus sub-clinical deficiency diagnosis in ruminant. Blood samples stored up to 48 hours were incubated with 32p at different periods and temperatures. There was no effect of storage time and the greatest 32p uptake values were obtained with incubation over 2 hours at 38 to 500C. (author)

Abstract (Portuguese)

Com o intuito de se estudar metodos para o diagnostico de deficiencia sub clinica de fosforo em ruminantes, desenvolveu-se na Secao de Ciencias Animais do CENA uma tecnica ''in vitro'' baseada na incorporacao de 32p pelos eritrocitos. Amostras de sangue foram incubadas com fosforo radioativo apos diferentes periodos de estocagem e por tempo e temperaturas variadas. Observou-se que o sangue pode ser estocado por 48 horas e deve ser incubado por 2 a 3 horas a uma temperatura entre 38 e 500C. (autor)

Additional details

Additional titles

Original title (Portuguese)
Determinacao 'in vitro' da taxa de incorporacao de

Publishing Information

Imprint Title
Proceedings of the 2. General Congress of Nuclear Energy - v.3
Imprint Pagination
498 p.
Journal Page Range
p. 349-352.
Report number
INIS-BR--1147(v.3)

Conference

Title
2. General Congress of Nuclear Energy.
Dates
24-29 Apr 1988.
Place
Rio de Janeiro, RJ (Brazil).

INIS

Country of Publication
Brazil
Country of Input or Organization
Brazil
INIS RN
19067396
Subject category
S60: APPLIED LIFE SCIENCES;
Resource subtype / Literary indicator
Conference
Descriptors DEI
DIAGNOSIS; ERYTHROCYTES; HEMIC DISEASES; IN VITRO; PHOSPHORUS 32; RUMINANTS; TEMPERATURE DEPENDENCE; TIME MEASUREMENT; UPTAKE
Descriptors DEC
ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; DAYS LIVING RADIOISOTOPES; DISEASES; ISOTOPES; LIGHT NUCLEI; MAMMALS; MATERIALS; NUCLEI; ODD-ODD NUCLEI; PHOSPHORUS ISOTOPES; RADIOISOTOPES; VERTEBRATES

Optional Information

Notes
Imprint:Anais do 2. Congresso Geral de Energia Nuclear - v.3.