Published December 1, 2017 | Version v1
Journal article

Chemically synthesized silver nanoparticles as cell lysis agent for bacterial genomic DNA isolation

  • 1. Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat-13, Assam (India)

Description

Silver nanoparticles (AgNPs) have seen a recent spurt of use in varied fields of science. In this paper, we showed a novel application of AgNP as a promising microbial cell-lysis agent for genomic DNA isolation. We utilized chemically synthesized AgNPs for lysing bacterial cells to isolate their genomic DNA. The AgNPs efficiently lysed bacterial cells to yield good quality DNA that could be subsequently used for several molecular biology works. (paper)

Availability note (English)

Available from http://dx.doi.org/10.1088/2043-6254/aa92ba

Additional details

Identifiers

Publishing Information

Journal Title
Advances in Natural Sciences. Nanoscience and Nanotechnology (Online)
Journal Volume
8
Journal Issue
4
Journal Page Range
[6 p.]
ISSN
2043-6262

INIS

Country of Publication
United Kingdom
Country of Input or Organization
International Atomic Energy Agency (IAEA)
INIS RN
49090957
Subject category
S77: NANOSCIENCE AND NANOTECHNOLOGY;
Descriptors DEI
CHEMICAL PREPARATION; DNA; LYSIS; MOLECULAR BIOLOGY; NANOPARTICLES; SILVER
Descriptors DEC
ELEMENTS; METALS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PARTICLES; SYNTHESIS; TRANSITION ELEMENTS