Fluorometric determination of ciprofloxacin using molecularly imprinted polymer and polystyrene microparticles doped with europium(III)(DBM)₃phen

The authors describe a microparticle-based system for the detection of the fluoroquinolone antibiotic ciprofloxacin. The method is using the tris(dibenzoylmethane)(1,10-phenanthroline)europium(III) luminophore in polystyrene microparticles along with a molecularly imprinted polymer (MIP) for ciprofloxacin. If ciprofloxacin is captured by the MIP, it quenches the fluorescence of the luminophores. Fluorescence drops linearly in the 0.5–100 μg L⁻¹ ciprofloxacin concentration range, and the detection limit is 92 ng L⁻¹. The method was applied to the analysis of fish samples to assess the analytical performance of the probe. Recoveries ranged from 85.4 to 86.6%, and relative standard deviations between 2.1 and 3.9% (for n = 5).

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