Micro-electrolytic radioiodination of polypeptide hormones to high specific activity

Electrolytic radioiodination employs a d.c. constant current or constant voltage source to generate an active radioiodine species, at a large surfaced platinum anode, to label tyrosine and histidine-containing compounds. This simple micro-electrolytic method, under carefully controlled conditions of pH, temperature, reaction time, current and anode potential has been shown to be reproducible and to yield labelled hormone preparations of high specific activity, which retain biological as well as immuno-logical activity. A new platinum microcell was designed for this purpose. 8author)