Published 1985
| Version v1
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Radiommunoassay for triiodothyronine in serum. Development of the solid phase technic and comparison with two liquid phase RIA systems: the polyethylene glycol (PEG) and double antibody methods
Creators
- 1. Instituto de Pesquisas Energeticas e Nucleares, Sao Paulo (Brazil)
Description
A solid phase radioimmunoassay (RIA) system for triiodothyronine (T3) was established by immobilizing triiodothyronine antibodies on the inner wall of reaction tubes. The antibody-coated tubes were made via reaction of antibody with glutaraldeyde residue pre coated on the inner wall of the tubes by alkaline self-polimerization. The quality of the coated tubes was tested through its performance in RIA methodology, by analysing the following RIA parameters: minimum detectable dose (MMD), nonspecific binding (NSB), X 50%, slope of the standard curve, intra and inter assay precision, accuracy of the method and figure of merit. The quality and characteristics of the reagents used in the RIA were analysed. (M.A.C.)
Availability note (English)
MF available from INIS under the Report Number.Abstract (Portuguese)
Estabeleceu-se um sistema de radioimunoensaio de hormonio triiodotironina (T3) pela tecnica de 'Fase Solida', imobizando-se anticorpo anti-T3 em tubos de polipropileno pre recobertos com glutaraldeido polimerizado em meio alcalino. Avaliou-se a qualidade dos tubos recobertos pelo seu desempenho metodologico no radioimunoensaio (RIE), analisando-se: a dose minima detectavel, a ligacao inespecifica ('NSB'), 'X50%', inclinacao da curva ('Slope'), precisao intra e inter ensaio, exatidao do medoto e digito de merito. Avaliou-se a qualidade e as caracteristicas dos reagentes utilizadas nos RIES. (M.A.C.)Files
18031942.pdf
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Additional details
Additional titles
- Original title (Portuguese)
- Radioimunoensaio do hormonio triiodotironina (T
Publishing Information
- Imprint Pagination
- 119 p.
- Report number
- INIS-BR--589
INIS
- Country of Publication
- Brazil
- Country of Input or Organization
- Brazil
- INIS RN
- 18031942
- Subject category
- S62: RADIOLOGY AND NUCLEAR MEDICINE;
- Resource subtype / Literary indicator
- Thesis
- Descriptors DEI
- ANTIGEN-ANTIBODY REACTIONS; BLOOD SERUM; COMPARATIVE EVALUATIONS; IODINE 125; RADIOIMMUNOASSAY; TRIIODOTHYRONINE
- Descriptors DEC
- BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD PLASMA; BODY FLUIDS; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; EVALUATION; HORMONES; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTO; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MATERIALS; NUCLEI; ODD-EVEN NUCLEI; PEPTIDE HORMONES; RADIOISOTOPES; THYROID HORMONES; TRACER TECHNIQUES