Effects of irradiation on expression of IL-3 and IL-3Rα in Dami cells
- 1. State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical Univ., Chongqing (China)
Description
Objective: To analyse the cell proliferation, cell cycle and the expression of IL-3, IL-3Rα in Dami cells after exposure to 60Co γ rays. Methods: After Dami cells were exposed to 60Co γ rays, the cell cycle and proliferation of Dami cells were analyzed by means of MTT and flow cytometry. The IL-3 and the IL- 3Rα expressions were detected by RT-PCR at 24 h after irradiation. Results: The irradiated Dami cells showed dose-dependent proliferative inhibition and G0/G1 block. Expressions of IL-3 and IL-3Rα mRNA in Dami after irradiation at doses of 5, 10, and 15 Gy were significantly lower than those in normal Dami cells (P<0.05). Their expressions showed reverse correlation with the dose. Conclusions: γ rays irradiation change the cell cycle, inhibits cell proliferation, IL-3 and IL-3Rα mRNA expression, suggesting that the abnormal expressions of IL-3 and IL-3Rα in Dami cells are involved in the injury of hemopoiesis by different doses of 60Co γ ray irradiation. (authors)
Additional details
Publishing Information
- Journal Title
- Chinese Journal of Radiological Medicine and Protection
- Journal Volume
- 27
- Journal Issue
- 5
- Journal Page Range
- p. 430-432
- ISSN
- 0254-5098
INIS
- Country of Publication
- China
- Country of Input or Organization
- China
- INIS RN
- 40060344
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Descriptors DEI
- BLOOD FORMATION; BONE MARROW CELLS; CELL CYCLE; CELL FLOW SYSTEMS; CELL PROLIFERATION; COBALT 60; DOSE-RESPONSE RELATIONSHIPS; GAMMA RADIATION; INHIBITION; IRRADIATION; LYMPHOKINES; MESSENGER-RNA; POLYMERASE CHAIN REACTION; RADIATION INJURIES
- Descriptors DEC
- ANIMAL CELLS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL EFFECTS; BIOLOGICAL RADIATION EFFECTS; COBALT ISOTOPES; CONNECTIVE TISSUE CELLS; DISEASES; ELECTROMAGNETIC RADIATION; GENE AMPLIFICATION; GROWTH FACTORS; INJURIES; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; IONIZING RADIATIONS; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; MINUTES LIVING RADIOISOTOPES; MITOGENS; NUCLEI; NUCLEIC ACIDS; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; PROTEINS; RADIATION EFFECTS; RADIATIONS; RADIOISOTOPES; RNA; SOMATIC CELLS; YEARS LIVING RADIOISOTOPES
Optional Information
- Notes
- 3 tabs., 12 refs.