Prophylactic Effects of DL-β Hydroxybutyrate against Hepatic Cellular Senescence Induced by D-galactose or γ-irradiation via Partial Modulation of Antioxidants and Trace Elements in Male Rats

Aging of cells is a normal feature but, it is important to delay this process and improve the life style. The present work aims at investigating the possible potential inhibitory effect of DL-β-hydroxybutyrate (βOHB) against hepatic cellular senescence induced by D-galactose or γ-irradiation in rats based on antioxidant and certain trace elements estimation in the liver. Six groups of male rats were used as follows: the control, irradiated group (acute dose 5 Gy, 2 weeks), D-galactose (150 mg/kg b.wt, 6 weeks), β OHB (72.8 mg/kg b.wt, 14 days), γ-irradiation plus β HB, and D-galactose plus βOHB. Results show that neither βOHB nor D-galactose administration had a significant effect relative to the normal control value on Hb, MCV, and RBCs. βOHB along with D-galactose or γ-irradiation significantly increased G6PD activity compared with the control group. In D-galactose, or γ-irradiation groups, liver MDA levels and SOD activity were significantly increased. Meanwhile, NO and GSH levels were significantly increased in the γ-irradiation group relative to the normal control levels. The findings show that β OHB alleviated hematological, antioxidant alterations and modulated the change in Cu, Fe, and Zn elements in D-galactose or γ-irradiation group. The results concur well with histological alteration in our previous findings. It could be concluded that these findings highlight a role for βOHB as a potent protective agent against hepatic cellular senescence associated liver injury through enhancing RBCs G6PD activity, reduction of oxidative stress and partial modulation of trace elements