Published March 1984 | Version v1
Journal article Metadata-only

Fluorometric analysis of the formation and repair of DNA breaks in irradiated cells

Creators

  • 1. Akademiya Meditsinskikh Nauk SSSR, Obninsk. Nauchno-Issledovatel'skij Inst. Meditsinskoj Radiologii

Description

A study was made of the dependence of the fluorescence of ethidium bromide upon NaOH concentration after staining of single- and double-strand dna in cell lysates was demonstrated. The method of fluorometry was used to study the dose dependence of a change in the share of double-stranded dna in the irradiated thymocytes and Ehrlich ascites carcinoma cells which permitted to de etermine the appearance and repair of dna breaks in these cells

Additional details

Additional titles

Original title (Russian)
Флуориметрический анализ появления и репарации разрывов В DNK облученных клеток

Publishing Information

Journal Title
Radiobiologiya
Journal Volume
24
Journal Issue
2
Series
Radiobiologiya.
Journal Page Range
154-157
ISSN
0033-8192

INIS

Country of Publication
Russian Federation
Country of Input or Organization
USSR
INIS RN
16038144
Subject category
S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
Descriptors DEI
ASCITES TUMOR CELLS; BIOLOGICAL REPAIR; DNA; EHRLICH ASCITES TUMOR; FLUORESCENCE SPECTROSCOPY; GAMMA RADIATION; IN VITRO; MICE; STRAND BREAKS; THYMOCYTES; TIME DEPENDENCE; TRANSPLANTS
Descriptors DEC
ANIMAL CELLS; ANIMALS; BIOLOGICAL RECOVERY; DISEASES; ELECTROMAGNETIC RADIATION; EMISSION SPECTROSCOPY; EXPERIMENTAL NEOPLASMS; IONIZING RADIATIONS; MAMMALS; NEOPLASMS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; RADIATIONS; RODENTS; SOMATIC CELLS; SPECTROSCOPY; TUMOR CELLS; VERTEBRATES