Fluorescent, thermo-responsive biotin-P(NIPAAm-co-NDAPM)- b-PCL micelles for cell-tracking and drug delivery
- 1. Key Laboratory of Biomedical Polymers of Ministry of Education and Department of Chemistry, Wuhan University, Wuhan 430072 (China)
Description
An amphiphilic, biotinylated poly(N-isopropylacrylamide-co-N-(3-dimethylamino propyl)methacrylamide)-block- poly(ε-caprolactone) (biotin-P(NIPAAm-co-NDAPM)- b-PCL) block copolymer was synthesized. The cytotoxicity study showed that the copolymer exhibited no apparent cytotoxicity. In aqueous solution, biotin-P(NIPAAm-co-NDAPM)- b-PCL copolymer was able to self-assemble into micelles of around 60 nm in diameter with a critical micellar concentration (CMC) of 36 mg l-1. Biotin-P(NIPAAm- co-NDAPM)-b-PCL micelles were thermo-responsive and the cloud point temperature was at 36.5 deg. C. The fluorescent group, fluorescein isothiocyanate (FITC) was further introduced to label the biotin-P(NIPAAm-co-NDAPM)- b-PCL copolymer. A cell internalization experiment was conducted and it was found that the fluorescent micelles could be internalized into the cells. The drug release behavior of drug-loading micelles was also examined and the drug-loaded biotin-P(NIPAAm-co-NDAPM)- b-PCL micelles showed slow drug release at 27 deg. C and fast drug release at 37 deg. C
Additional details
Identifiers
- DOI
- 10.1088/0957-4484/18/50/505101;
- PII
- S0957-4484(07)51440-6;
Publishing Information
- Journal Title
- Nanotechnology (Print)
- Journal Volume
- 18
- Journal Issue
- 50
- Journal Page Range
- p. 505101
- ISSN
- 0957-4484
INIS
- Country of Publication
- United Kingdom
- Country of Input or Organization
- International Atomic Energy Agency (IAEA)
- INIS RN
- 39040260
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- AQUEOUS SOLUTIONS; BIOTIN; COPOLYMERS; DELIVERY; DRUGS; FLUORESCEIN; ISOTHIOCYANATES; TEMPERATURE DEPENDENCE; TEMPERATURE RANGE 0273-0400 K; TOXICITY
- Descriptors DEC
- AROMATICS; AZOLES; CARBONIC ACID DERIVATIVES; CARBOXYLIC ACIDS; DISPERSIONS; DYES; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; HOMOGENEOUS MIXTURES; HYDROXY ACIDS; HYDROXY COMPOUNDS; IMIDAZOLES; MIXTURES; NITROGEN COMPOUNDS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANIC POLYMERS; ORGANIC SULFUR COMPOUNDS; PHENOLS; POLYMERS; POLYPHENOLS; SOLUTIONS; TEMPERATURE RANGE; VITAMIN B GROUP; VITAMINS