Published August 1988 | Version v1
Journal article Metadata-only

Impaired repair of UVC-induced DNA damage in L5178Y-R cells: DNA unwinding studies with the use of 1-β-D-arabinofuranosyl cytosine

Creators

  • 1. Institute of Nuclear Chemistry and Technology, Warsaw (Poland)
  • 2. Swedish Univ. of Agricultural Sciences, Uppsala. Dept. of Radioecology

Description

L5178Y-R (LY-R) and L5178Y-S (LY-S) mouse lymphoma cells differ in sensitivity to UVC radiation (D0: 2.8 and 9.0 J/m2-respectively, for cells exposed in Fischer's medium). We used these cells and a DNA unwinding technique in conjunction with 1-β-D-arabinosyl cytosine to determine DNA strand breaks accumulating as a result of enzymatic incision during DNA repair. Following UVC exposure DNA strand break accumulation was observed in LY-S cells but not in LY-R cells. The repair defect in LY-R cells is accompanied by a delayed recovery of [3H]thymidine incorporation. (author)

Additional details

Publishing Information

Journal Title
Photochemistry and Photobiology
Journal Volume
48
Journal Issue
2
Series
Photochem. Photobiol.
Journal Page Range
201-204
ISSN
0031-8655
CODEN
PHCBA

INIS

Country of Publication
United Kingdom
Country of Input or Organization
United Kingdom
INIS RN
20002327
Subject category
S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
Descriptors DEI
CELL CULTURES; DNA REPAIR; LYMPHOMAS; MICE; PHOTOSENSITIVITY; STRAND BREAKS; TUMOR CELLS; ULTRAVIOLET RADIATION
Descriptors DEC
ANIMAL CELLS; ANIMALS; BIOLOGICAL RECOVERY; BIOLOGICAL REPAIR; DISEASES; ELECTROMAGNETIC RADIATION; MAMMALS; NEOPLASMS; RADIATIONS; RODENTS; SENSITIVITY; VERTEBRATES